When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

Gene-Analysis Studies Advanced by Use of Low-Volume High-Throughput Real-Time PCR This article describes three gene-analysis studies using the Roche LightCycler® 1536 Real-Time PCR System (Figure 1).

During the early stages of the coronavirus disease 2019 (COVID-19), rapid testing was imperative for public health policymakers and health care workers in order to determine where and what measures ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

Journal of Plant Pathology, Vol. 97, No. 2 (July 2015), pp. 259-263 (5 pages) Leifsonia (Clavibacter) xyli subsp. xyli (Lxx) is the etiological agent of ratoon stunting disease (RSD), one of the most ...